Molecular Dispersion of Starch as a Crucial Parameter during Size-Exclusion Chromatography

Size Exclusion Chromatography of Polymers

In the characterization of polymers, size-exclusion chromatography (SEC) has become a standard technique for determining molar mass averages andmolar mass distributions (MMD) of polymers. The principle of SEC is easily understood: owing to limited accessability of the pore volume within the particles of the column packing, polymer molecules are separated according to their hydrodynamic volumes,...

A Deadenylase Assay by Size-Exclusion Chromatography

The shortening of the 3'-end poly(A) tail, also called deadenylation, is crucial to the regulation of mRNA processing, transportation, translation and degradation. The deadenylation process is achieved by deadenylases, which specifically catalyze the removal of the poly(A) tail at the 3'-end of eukaryotic mRNAs and release 5'-AMP as the product. To achieve their physiological functions, all dea...

Effects of Single - Screw Extrusion Cooking on Starch as Measured by Aqueous High - Performance Size - Exclusion Chromatography " 2 ' 3

Cereal Chem. 67(6):529-532 The water solubilization and apparent molecular weights of starch evident, the HPSEC-determined water solubility increased with decreasing polymers from extruded corn starch and extruded decorticated sorghum extrusion moisture. Decreased water solubility of corn starch, however, flours were characterized by aqueous high-performance size-exclusion was caused by extrusi...

Measurement of Albuminuria with Size-exclusion Chromatography

Size exclusion chromatography does not require pores.

Separation of macromolecules on the basis of their molecular weight by size exclusion chromatography has long been considered to be caused by the geometry-dependent partition of macromolecules between a continuous phase and the porous interior of a gel or cross-linked bead. The volume of a pore accessible to a solute is limited by its relative dimensions, so larger molecules will have access to...